High-throughput analysis of TUNEL-stained sperm using image cytometry
نویسندگان
چکیده
منابع مشابه
A High Throughput and High Content Analysis of Cell Death Processes Using Microfluidic Image Cytometry (fic)
Microfluidic systems have significant implications in the field of in vitro cell-based assays and drug screening by virtue of their automation and high content screening capabilities. Here, we present our recent efforts to develop a microfluidic platform for high throughput and high content analysis of cell death event. A group of microfluidic devices for monitoring various cell death parameter...
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Flow cytometry (FCM) software packages from R/Bioconductor, such as flowCore and flowViz, serve as an open platform for development of new analysis tools and methods. We created plateCore, a new package that extends the functionality in these core packages to enable automated negative control-based gating and make the processing and analysis of plate-based data sets from high-throughput FCM scr...
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An association between DNA fragmentation in sperm determined by the terminal deoxynucleotidyl transferase [TdT]-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) assay and the incidence of reproductive failure has been reported, either using flow cytometry or optical microscopy. However, the results obtained using each of these two approaches are different. Since there is a re...
متن کاملFragmentation of sperm DNA using the TUNEL method.
OBJECTIVES To establish the validity of the TUNEL assay in determining sperm DNA fragmentation, the relationship between the degree of fragmentation and the seminal parameters and the sample needed to conduct the test. MATERIAL AND METHODS We used semen samples from healthy fertile men (n=33), patients who consulted for infertility with a prescription for the TUNEL assay (n=77) and patients w...
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Phage display libraries represent a relatively easy way to generate binding ligands against a vast number of different targets. Although in principle, phage display selection should be amenable to automation, this has not yet been described and present selection protocols are far from high throughput. We have examined the selection process in a systematic approach and attempted to automate each...
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ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2017
ISSN: 1552-4922
DOI: 10.1002/cyto.a.23164